Author

Thiago Luiz Alves e Silva

National Institutes of HealthORCID 0000-0002-8520-5405Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:cell type annotation· 2 Research Focus:host-pathogen & immune response· 2 Assay:imaging· 2 Organism:mouse· 2 Research Focus:cell signaling & trafficking· 1 Assay:proteomics· 1 Organism:virus· 1

Frequent Co-Authors

Benjamin LiffneriD· 3 shared Joel Vega-RodrígueziD· 3 shared Sabrina AbsaloniD· 3 shared Alexis KaushanskyiD· 1 shared Ana Beatriz F. BarlettaiD· 1 shared Bianca M. NagataiD· 1 shared Carolina V. Barillas-MuryiD· 1 shared Cindi L. SchwartziD· 1 shared

Activity by Year

2024-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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3 shared papersMicrobiology

Benjamin LiffnerORCID

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3 papers

Recent·Most cited

High-Resolution Proteomics Unveils Salivary Gland Disruption and Saliva-Hemolymph Protein Exchange in Plasmodium-Infected Mosquitoes

Thiago Luiz Alves e Silva, Sachie Kanatani, Ana Beatriz F. Barletta, Cindi L. Schwartz, Benjamin Liffner, Octávio A. C. Talyuli, Janet J. Olivas, Bianca M. Nagata, Zarna Rajeshkumar Pala, Tales Vicari Pascini, Derron A. Alves, Ming Zhao, Motoshi Suzuki, Lilian Patrick Dorner, Friedrich Frischknecht, , Joel Vega-Rodríguez

This study uses high-resolution proteomics to show that Plasmodium infection disrupts mosquito salivary glands, causing exchange of proteins between hemolymph and saliva.

Nature Communications · Nov 20, 2025Peer-reviewedMicrobiology

Unlocking new understanding of Plasmodium sporozoite biology with expansion microscopy

Benjamin Liffner, Thiago Luiz Alves e Silva, Elizabeth K. K. Glennon, Veronica I. Primavera, Elaine Hoffman, Alexis Kaushansky, Joel Vega-Rodríguez, Sabrina Absalon

This study uses expansion microscopy to investigate sporozoite biology in malaria parasites, revealing new insights into rhoptry function and identifying a key protein involved in invasion.

bioRxiv · Apr 9, 2025 · PreprintMicrobiology

Mosquito Tissue Ultrastructure-Expansion Microscopy (MoTissU-ExM) enables ultrastructural and anatomical analysis of malaria parasites and their mosquito.

Benjamin Liffner, Thiago Luiz Alves e Silva, Joel Vega-Rodríguez, Sabrina Absalon

The paper introduces MoTissU-ExM, a method to expand mosquito tissues for visualizing malaria parasites, enabling ultrastructural analysis of both host and parasite structures.

BMC Methods · Oct 7, 2024Peer-reviewedCell Biology