Resolving hidden stoichiometries in Bacterial proteasome activator (Bpa)-substrate complexes by cryo-EM and charge detection mass spectrometry
Bradley T. V. Davis, Adwaith B. Uday, Anisha Haris…·Siavash Vahidi
This study uses cryo-EM and charge detection mass spectrometry to determine the stoichiometry and structure of Bpa-substrate complexes in Mtb, revealing an undecameric apo form and a Bpa12:HspR2 complex.