BiochemistrybioRxivPreprint
Helical transition of the bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation
Chhandosee Ganguly, Lindsie Martin, Swarmistha Devi Aribam…·Rakhi Rajan
This study investigates how the bridge helix (BH) of Cas12a regulates R-loop formation and RuvC activation through conformational changes, revealing structural mechanisms for improving Cas12a specificity.
DOI 10.1101/2025.01.09.632262