Ruth V. Nichols

This study maps early postnatal DNA methylation dynamics in the mouse brain, revealing subtype-specific methylation changes and their disruption in Rett syndrome.

The paper introduces sciMETv3, a high-throughput single-cell DNA methylation profiling method, achieving 100-fold higher cell throughput and compatibility with multimodal assays.

sciMET-cap reduces sequencing burden for single-cell methylation analysis while enabling accurate cell type assignment and near-complete methylome reconstruction.