Targeted genome fragmentation with CRISPR/Cas9 improves hybridization capture, reduces PCR bias, and enables efficient high-accuracy sequencing of small targets
Daniela Nachmanson, Shenyi Lian, Elizabeth K. Schmidt, Michael J. Hipp, Kathryn Terese Baker, Yuezheng Zhang, Maria Tretiakova, Kaitlyn J. Loubet-Senear, Brendan F. Kohrn, Jesse J. Salk, Scott R. Kennedy, Rosa Ana Risques
This paper introduces CRISPR-DS, a targeted sequencing technique that improves hybridization capture, reduces PCR bias, and enables detection of ultra-low frequency mutations.
Genome Research · Oct 1, 2018Peer-reviewedGenomics