PolyA tail segmentation improves the stability of the template DNA and increases the translatability of in vitro transcribed mRNA
Tomasz Śpiewla, Karol Czubak, Zofia Pilch, Marek R. Baranowski, Paweł Szczepan Krawczyk, Kamila Affek, Wiktor Antczak, Marta Szulc-Gasiorowska, Sebastian Chmielinski, Seweryn Mroczek, Michał Brouze, Dominika Nowis, Jakub Gołąb, Andrzej Dziembowski, Jacek Jemielity, Joanna Kowalska
The paper develops segmented polyA tail designs to improve DNA stability and mRNA translatability, finding that longer segmented tails significantly enhance protein production.
Nucleic Acids Research · Dec 2, 2025Peer-reviewedMolecular Biology