The paper develops segmented polyA tail designs to improve DNA stability and mRNA translatability, finding that longer segmented tails significantly enhance protein production.

Nucleic Acids Research · Dec 2, 2025Peer-reviewedMolecular Biology

Whole-miRNome sequencing (WMS) - a panel for targeted sequencing of all human miRNA genes

Paulina Galka-Marciniak, Martyna Olga Urbanek-Trzeciak, Daniel Kuźnicki, Natalia Szóstak, Adrian Tire, Paulina Maria Nawrocka, Katarzyna Chojnacka, Malwina Suszyńska, Katarzyna Klonowska, Karol Czubak, Magdalena Machowska, Anna Philips, Konstantin Maksin, Laura Susok, Michael S. Sand, , Piotr Kozłowski

The paper introduces WMS, a targeted sequencing method for all human miRNA genes, identifying mutations and copy number alterations in cancer samples.

Nucleic Acids Research · Aug 6, 2025Peer-reviewedGenomics

Chemical circularization of in vitro transcribed RNA opens new avenues for circular mRNA design

Małgorzata Wąsińska-Kałwa, Adam Mamot, Karol Czubak, Katarzyna Frankowska, Adam A. Rajkiewicz, Tomasz Śpiewla, Marcin Warmiński, Zofia Pilch, Marta Szulc-Gasiorowska, Kacper Siekan, Andrzej Dziembowski, Dominika Nowis, Jakub Gołąb, Joanna Kowalska, Jacek Jemielity

This paper introduces a chemical method to circularize in vitro transcribed RNA, producing chemically circularized RNAs (chem-circRNAs) with high efficiency and compatibility with modifications.

Nature Communications · Jul 12, 2025Peer-reviewedBiochemistry

Global increase in circRNA levels in myotonic dystrophy

Karol Czubak, Katarzyna Taylor, Agnieszka Piasecka, Krzysztof Sobczak, Katarzyna Kozlowska, Anna Philips, Saam Sedehizadeh, J. David Brook, Marzena Wojciechowska, Piotr Kozłowski

This study shows increased global circRNA levels in myotonic dystrophy type 1, identifies circRNAs associated with disease severity, and links circRNA levels to muscle weakness and alternative splicing changes.

Frontiers in Genetics · Jul 18, 2019Peer-reviewedMolecular Biology