Diego Alignani

This study identifies transcriptional changes in stromal and endothelial cells during MGUS to myeloma progression and treatment response, revealing a tumor-permissive BME signature that is modulated by therapy.

This study uses single-cell RNA sequencing to analyze EDA-CAR-T cell dynamics in the tumor microenvironment, revealing temporal reprogramming toward dysfunction and identifying immunosuppressive pathways that impair therapeutic efficacy in solid tumors.

This study uses single-cell multiomics to analyze CAR T cell dynamics in multiple myeloma, identifying regulatory mechanisms affecting persistence and dysfunction.

The paper identifies epigenetic regulators of fibrotic transformation in cardiac fibroblasts using bulk and single-cell CRISPR screens and shows KAT5 inhibition reduces fibrotic responses in human fibroblasts.

This paper identifies SLU7 as a pan-cancer target, showing its knockdown induces genomic instability and enhances immunogenicity, with effective tumor suppression in murine models.

This study identifies novel RAB13+ endothelial and profibrotic mesenchymal cells in aged human bone marrow using single-cell RNA sequencing and spatial transcriptomics.

This study shows gene therapy can restore the transcriptional program of hematopoietic stem cells in Fanconi Anemia patients, making them resemble healthy donor cells.

This study uses single-cell RNA sequencing to analyze changes in hematopoietic stem and progenitor cells during aging and myeloid malignancies, identifying altered gene expression and regulatory networks.

This study uses CRISPR screens and Perturb-Seq to analyze 550 chromatin factors in haematopoiesis and AML, revealing lineage-specific roles and therapeutic implications.