David Lara‐Astiaso

Diversity and evolution of chromatin regulatory states across eukaryotes

The paper profiles histone post-translational modifications across eukaryotic lineages to assess conservation of chromatin states, finding conserved euchromatin states but diverse repressive heterochromatin configurations.

Spatially resolved, multimodal in vivo Perturb-seq using antibody-based cell hashing

This paper introduces PerturbSpace, a method combining CRISPR perturbations with spatially hashed single-cell multiomics to study gene function in tissue architecture.

Identification of epigenetic regulators of fibrotic transformation in cardiac fibroblasts through bulk and single-cell CRISPR screens

The paper identifies epigenetic regulators of fibrotic transformation in cardiac fibroblasts using bulk and single-cell CRISPR screens and shows KAT5 inhibition reduces fibrotic responses in human fibroblasts.

Adenine DNA methylation associated to transcription is widespread across eukaryotes

The paper profiles 6mA methylation in 18 eukaryotes and finds it is linked to transcription and associated with H3K4me3-enriched nucleosomes, but is lost in major multicellular lineages.

Differences between in vivo and ex vivo models drive divergent perturbation outcomes

The paper compares perturbation outcomes in in vivo and ex vivo hematopoietic stem cell models, finding systematic biases and challenges in predicting in vivo effects from ex vivo data.

Delayed Trp53 activation protects Dnmt3a-mutant hematopoietic stem cells from inflammatory attrition.

This study shows that DNMT3A R882H mutant HSCs resist inflammatory stress through delayed p53 activation, leading to clonal expansion and increased AML risk.

Systematic functional screening of chromatin factors identifies strong lineage and disease dependencies in normal and malignant haematopoiesis

This study uses CRISPR screens and Perturb-Seq to analyze 550 chromatin factors in haematopoiesis and AML, revealing lineage-specific roles and therapeutic implications.