Shosei Imai

This paper introduces a biosensor-based method for single-molecule enzyme activity profiling without enzyme-specific substrates, enabling absolute quantification of active enzyme molecules in cellular and circulating samples.

The paper introduces PinkyCaMP, a new red fluorescent calcium sensor with improved brightness, photostability, and compatibility with optogenetics and neurotransmitter imaging.

The paper develops high-performance green and red fluorescent biosensors for pyruvate with large fluorescence intensity changes and varying affinities, enabling multicolor imaging in mammalian cells.