Robert E. Campbell

The paper develops a new calcium indicator, HiCaRI, based on the photostable mStayGold protein, showing improved brightness and photostability compared to existing GFP-based indicators.

This paper introduces a biosensor-based method for single-molecule enzyme activity profiling without enzyme-specific substrates, enabling absolute quantification of active enzyme molecules in cellular and circulating samples.

The paper develops a photostable pH biosensor called serapH using mStayGold as a scaffold and introduces a screening method for pH-sensitive FPs in bacterial colonies.

The paper introduces OCaMP, an orange fluorescent calcium indicator that improves sensitivity and performance compared to existing green and red indicators for neural imaging.

The paper introduces PinkyCaMP, a new red fluorescent calcium sensor with improved brightness, photostability, and compatibility with optogenetics and neurotransmitter imaging.

This paper develops excitation ratiometric fluorescent protein biosensors for citrate, enabling quantitative analysis of citrate dynamics in different cellular compartments and populations.

The paper introduces three genetically encoded tools to specifically ablate excitatory and inhibitory synapses using E3 ligase-dependent degradation mechanisms.

The paper develops high-performance green and red fluorescent biosensors for pyruvate with large fluorescence intensity changes and varying affinities, enabling multicolor imaging in mammalian cells.

This paper introduces two new far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, characterized for their Ca2+ sensitivity and performance in neuronal imaging.

This paper develops a photocaged lactate derivative to locally perturb lactate concentrations and study its roles in metabolism and signaling.

The paper develops T-GECO1, a blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon brightness for improved neuronal activity imaging.

The paper shows lactate-dependent glycolysis shifts produce D-serine, linking astrocyte metabolism to synaptic and cognitive functions through CB1 receptor activation.

This paper develops improved fluorescent biosensors for monitoring extracellular and intracellular L-lactate in mammalian cells, showing enhanced sensitivity and response kinetics.

This paper develops a genetically encoded fluorescent biosensor for extracellular L-lactate and demonstrates its use in imaging in cultured mammalian cells and brain tissue.

This paper develops improved photocleavable proteins with faster and more efficient dissociation for better optogenetic control.

The paper introduces two improved near-infrared calcium indicators for better neuronal activity imaging in vivo.

The paper develops two high-performance genetically encoded biosensors for citrate imaging in mammalian cells, showing their ability to detect both steady-state and perturbed citrate concentrations.

The paper develops a new genetically encoded Ca2+ indicator, mNG-GECO1, based on mNeonGreen, which is brighter than GCaMP6s and performs comparably in various imaging applications.

The paper develops all-optical electrophysiology tools to record subthreshold dynamics and excitability in mouse hippocampus during behavior, revealing brain-state dependent changes in neuronal activity.

The paper develops tools for wide-area mapping of neuronal activity in brain slices using optimized optogenetic tools and structured illumination.

This paper develops a FRET-based genetically encoded K+ indicator, KIRIN1, which detects K+ with high selectivity and enables real-time imaging in live cells.