Toru Komatsu

Biosensor-enabled single-molecule enzyme activity profiling for function-based molecular counting

This paper introduces a biosensor-based method for single-molecule enzyme activity profiling without enzyme-specific substrates, enabling absolute quantification of active enzyme molecules in cellular and circulating samples.

Topical Activatable Fluorescence Probes for Rapid Intraoperative Detection of Peritoneal Dissemination in High-Grade Serous Ovarian Carcinoma

This paper develops three topical fluorescence probes for intraoperative detection of peritoneal dissemination in high-grade serous ovarian carcinoma, showing high sensitivity and specificity in clinical and mouse models.

Early detection of pancreatic ductal adenocarcinoma by single-molecule profiling of pancreatic enzyme activities

This study develops a blood-based liquid biopsy platform using single-molecule enzyme activity profiling to detect pancreatic ductal adenocarcinoma with high specificity and moderate sensitivity across multiple stages.

Single-Molecule Activity Profiling of Glycosidase Proteoforms Using Water-Soluble Fluorogenic Probes

This study develops water-soluble fluorogenic probes for single-molecule glycosidase activity profiling in blood, revealing -mannosidase as a potential biomarker for liver injury.

Solid-Phase Synthesis of ProTide Fluorogenic Probes Enables Systematic Profiling of Carboxypeptidase Activity

This study develops fluorogenic ProTide probes to profile carboxypeptidase activities, showing elevated levels in pancreatic cancer patients.

Synthesis based on covalent capture and release (SCCR): a programmable strategy for automated preparation of protease-activatable molecules

This paper introduces a programmable chemical strategy called SCCR for automated synthesis of protease-activatable molecules, demonstrating its application in generating fluorogenic probes and ADC linkers.

Development of single-molecule enzyme activity assay for serine hydrolases using activity-based protein labeling probes

This paper develops a single-molecule enzyme activity assay for serine hydrolases using activity-based probes and demonstrates its application in detecting active granzyme B as a biomarker of liver damage.

Platform of single-molecule enzyme activity-based liquid biopsy for detection of pancreatic adenocarcinoma at early stages

This paper introduces a liquid biopsy platform that detects early-stage pancreatic adenocarcinoma by analyzing single-molecule protease/peptidase activity in blood samples.