Emmanuelle Nguyen

This paper develops an automated assay to quantify synuclein aggregate formation in human dopaminergic neurons using pS129-syn as a marker, showing dose- and time-dependent increases with PFFs and genetic factors affecting aggregate formation.

The paper describes a high-content imaging assay to screen small molecules that reduce cellular uptake of α-synuclein preformed fibrils in hiPSC-derived cells, identifying compounds that effectively lower intracellular PFF load.

This study uses a convolutional neural network to classify Parkinson's disease models from control cells with high accuracy using microscopy images.

The paper shows that combining α-synuclein fibrils with immune challenges causes Lewy body-like inclusions in human dopaminergic neurons, while cortical neurons do not form such inclusions.