Patrick J. Almhjell

This paper evolves noncanonical amino acid synthases in yeast to produce ncAAs from simple precursors, enabling scalable genetic code expansion.

uSort-M isolates and identifies protein variants from pooled libraries using automated sorting and long-read sequencing, achieving high recovery rates at lower cost.

The paper constructs a large-scale sequence-fitness dataset for enzyme engineering, revealing significant epistasis and highlighting challenges in predicting optimal variants.

This paper develops a continuous directed evolution platform called OrthoRep to generate diverse enzyme variants with promiscuous activities by evolving TmTrpB through multi-mutation pathways.