Fan Liang

NextPolish2 improves genome accuracy by fixing base errors in HiFi assemblies without overcorrections or haplotype switch errors.

This study generates haplotype-resolved assemblies and a variant benchmark for a Chinese monozygotic twin quartet using advanced sequencing and computational methods.

This paper introduces Quartet DNA reference materials to comprehensively evaluate germline variant calling accuracy across the genome, including difficult regions and batch effects.

NextDenovo corrects noisy Nanopore reads faster and more accurately than Canu and Necat; its maize and 35 human-genome assemblies are more contiguous, with fewer misassemblies, than Flye's.

The paper uses single-molecule long-read sequencing to identify structural variants driving high-altitude adaptation in Tibetans, revealing their role in gene regulation and biological functions.

The paper establishes high-confidence structural variant calls in an Asian individual using long-read sequencing technologies and validates them through PCR and Sanger sequencing.

This study compares genome assemblies from Pacbio HiFi and Oxford Nanopore ultralong reads in rice, finding that ONT provides better contiguity but more errors, while PB offers fewer errors but lower contiguity.

This study uses Oxford Nanopore long-read sequencing to precisely localize breakpoints in balanced translocations and inversions, achieving single-base resolution for some cases.

The paper presents a high-quality tung tree genome, identifies oil-related genes, and provides insights into genome evolution and oil biosynthesis mechanisms.

This paper develops a single-molecule optical mapping method for quantitative D4Z4 repeat analysis in FSHD, showing concordance with Southern blot and improved haplotype resolution.

DeepSignal detects DNA methylation sites from Nanopore sequencing reads using deep learning, achieving higher accuracy than HMM-based methods across multiple organisms and methylation types.