Roman Teo Oliynyk

This paper introduces a method using single-stranded DNA templates and bacteriophage RNA polymerases to produce functional RNAs in mammalian cells, demonstrating applications in CRISPR-based genome editing.

The paper demonstrates DNA cyclization efficiencies exceeding classical Jacobson-Stockmayer predictions by over threefold using optimized enzyme combinations.

This paper presents a rapid, low-cost method to produce circular DNA for expression in cell culture, demonstrating its effectiveness for prime editing guide RNA.

This paper assesses the population genetics consequences of preventive gene therapies for polygenic diseases using simulations, showing that allele heterogeneity does not impede therapy outcomes but may affect disease onset and risk over time.

This paper evaluates how using younger cases and older controls in genome-wide association studies can enhance discovery power for late-onset polygenic diseases.

This study uses simulations to assess how future gene therapy could reduce lifetime risk and delay onset of polygenic late-onset diseases.

This paper shows that genome-wide association studies miss heritability in late-onset diseases due to cohort biases, suggesting younger case-control designs improve discovery power.