Ramiro M. Perrotta

A Developmental Lectin-Glycan Program Enables Early Breast Cancer Dissemination and Metastatic Onset

This study identifies a developmental GAL1-glycan program that promotes early breast cancer dissemination and metastasis by reactivating mammary stem cell traits and epithelial plasticity.

Short single-stranded DNA oligonucleotides enable intracellular transcription of functional RNAs in mammalian cells

This paper introduces a method using single-stranded DNA templates and bacteriophage RNA polymerases to produce functional RNAs in mammalian cells, demonstrating applications in CRISPR-based genome editing.

Preventing escape and malfunction of recoded cells due to tRNA base changes

The paper shows recoded E. coli can escape faster due to tRNA base changes and introduces kill switches to prevent this.

Machine Learning and Directed Evolution of Base Editing Enzymes

This work designs precise base editors with reduced bystander editing using guide RNA libraries and protein language models, improving safety and targeting efficiency.