Andreas Walther

Programmable DNA Protonuclei Reveal Environmental Context on Protein Phase Separation

This paper introduces programmable DNA-based protonuclei to study protein phase separation, showing that environmental factors significantly influence FUS behavior.

RNA Organelles in DNA-based Artificial Cells Provide Spatial Aptamer Functions and Enhanced Signal Processing

This paper develops DNA-RNA artificial cells with RNA organelles that enable spatially encoded functions and signal processing through aptamer-based protein recruitment and environmental responsiveness.

DNA Flipping as Facile Mechanism for Transmembrane Signaling in Synthetic Cells

This paper demonstrates DNA flipping across lipid membranes in synthetic cells to enable transmembrane signaling and transcriptional activation.

Stable Synthetic Organelles from Aqueous Two-Phase Systemswith Access to the Cell Translation Machinery

This paper introduces dextran-lipid droplets as stable synthetic compartments that can be taken up by cells and access the translation machinery for mRNA-based protein production.

ATP-Powered Signaling between Synthetic and Living Cells

This paper describes a method to deliver DNA signals from synthetic cells to living cells using ATP-driven reactions, with potential applications in gene therapy and cancer treatment.

Synthetic Aptamer Mechanoreceptors Enable Cell-SpecificForce Sensing and Temporal Control via DNA Circuits

This paper introduces a DNA-based mechanosensing platform using aptamers to enable cell-specific force sensing and temporally controlled mechanoresponses through programmable DNA circuits.

Deep Learning Reaction Network: a machine learning framework for modeling time resolved data

The paper introduces DLRN, a deep learning framework that models time-resolved data to extract kinetic reaction networks, time constants, and amplitudes with performance comparable to or better than classical methods.

Nuclease-Resistant L-DNA Tension Probes Enable Long-TermForce Mapping of Single Cells and Cell Consortia

This paper introduces L-DNA tension probes that resist nuclease degradation, enabling long-term force mapping of single cells and migrating cell populations.