Tomás Pachano

The paper systematically identifies effector functions of pathogen ORFs across multiple pathways, revealing new roles for known effectors and uncharacterized proteins.

The paper uses synthetic enhancer engineering in mESCs to show that enhancer clusters increase transcriptionally productive enhancer-gene contacts through enhanced RNA Polymerase II pause release.

The paper shows that Polycomb repression during S/G2 phases restricts differentiation gene activation, allowing it to occur primarily in G1 phase in mouse ESCs.

The paper shows that poised enhancers' chromatin and 3D features are conserved in vivo in mouse and chicken embryos, and they play essential roles in developmental gene induction.

The paper shows that orphan CpG islands enhance the regulatory activity of poised enhancers and influence the responsiveness of target genes.