Kunihiko Nishino

This paper enhances Cas12a's trans-cleavage activity by fusing Sso7d, improving sensitivity and kinetics for nucleic-acid detection.

This study identifies and characterizes FoeAB, a type IV ABC transporter in Streptococcus pneumoniae that exports fosfomycin, using functional assays and cryo-EM structures.

This study presents crystal structures of Streptococcus pneumoniae PYK bound to nucleotides, revealing structural basis for GTP selectivity and its role in nucleotide homeostasis.

This paper investigates Arg717 substitutions in Escherichia coli AcrB, finding they increase resistance to macrolides and fluoroquinolones while decreasing resistance to novobiocin and cloxacillin.

The paper uses a convolutional neural network to classify antibiotic-resistant E. coli cells in TEM images based on morphological features, achieving 94% accuracy.