Ian Shen Hsu

This paper describes a method to efficiently generate heterothallic fission yeast strains using CRISPR-Cas9 and a mating type locus alteration.

The paper identifies functional short linear motifs in disordered regions of HOG signaling proteins and shows their quantitative impact on pathway output through experimental and computational approaches.

The paper shows that constant calcium stress causes stochastic nuclear localization pulses of Crz1 in yeast, proposing a model where time delays and stochasticity convert analog calcium signals into digital pulses.