Andrew P. May

This study profiles adult mouse spinal cord ependymal cells using single-cell RNA-sequencing, revealing a new mature state and dynamic maturation regulation in response to injury.

This study uses single-cell RNA-sequencing to analyze cell type-specific responses to young or aged blood in heterochronic parabiosis, identifying mitochondrial function as a key factor in rejuvenation.

This study compares rAAV production methods using different host cells, finding chemically and functionally distinct vectors with differences in PTMs, methylation, and potency.

The paper identifies IgnaviCas9, a hyperthermophilic Cas9 from an unculturable Ignavibacterium, which functions at higher temperatures than existing Cas9 variants and is applied in RNA-seq library preparation.

This study systematically characterizes genome editing in primary T cells, identifies proximal genomic insertions, and develops a machine learning model to predict CRISPR-Cas9 DNA repair outcomes.

This paper develops a non-viral CRISPR-Cas9 system for precise genome editing in human T cells, enabling therapeutic reprogramming of cell function and specificity.