GenomicsbioRxivPreprint
Correcting PCR amplification errors in unique molecular identifiers to generate absolute numbers of sequencing molecules.
Jianfeng Sun, Martin Philpott, Danson Shek Chun Loi…·Adam P. Cribbs
The paper demonstrates that PCR errors significantly affect UMI-based absolute counting in sequencing data and proposes a solution using homotrimeric nucleotide blocks to correct these errors.
2 citations