Maria J. Gomez-Lamarca

The paper investigates changes in CSL transcription complex behavior under Notch active conditions using single molecule microscopy, finding increased binding and exploratory behavior.

The paper reveals that Notch transcription hubs form dynamically in nuclei and confer memory, with only a third progressing to transcription, influenced by a second signal.

The paper uses an optogenetic approach to control Notch signaling and shows that the {Phi}W{Phi}P motif sequesters CSL in the cytoplasm, preventing target gene activation until NICD is released.