Gary Kleiger

This study uses pre-steady state kinetics and global data fitting to analyze CRL-dependent ubiquitylation, revealing kinetic parameters and catalytic efficiencies across different CRL systems and substrate contexts.

The paper identifies LRRC58 as a cysteine-responsive E3 ligase that regulates CDO1 stability via ubiquitylation, revealing a mechanism linking cysteine availability to proteasomal turnover.

This paper designs a protein inhibitor for UBE2D enzymes to study their role in ubiquitination, showing it reduces Ub degradation and proteotoxic stress in HeLa cells.