Brenda Arlene Schulman

Global Data Fitting Enables Kinetic Dissection of Cullin-RING Ligase-Catalyzed Ubiquitylation: From Substrate Priming to Poly-ubiquitin Chain Formation

This study uses pre-steady state kinetics and global data fitting to analyze CRL-dependent ubiquitylation, revealing kinetic parameters and catalytic efficiencies across different CRL systems and substrate contexts.

Targeting proteasome composition reprograms tumor-intrinsic inflammation through selective degradation of STAT1

This study identifies a small molecule targeting PSME4, which stabilizes STAT1 and reprograms tumor inflammation, suppressing tumor growth by altering cytokine signaling.

Multimodal substrate recruitment enables CTLH-MKLN1 E3 ligase to target N-, C-, and internal degrons

This study reveals how MKLN1's discoidin and Kelch domains recognize diverse degrons through multivalent interactions, enabling substrate recruitment by the CTLH-MKLN1 E3 ligase.

Structural basis for regulation of the proteasome 20S core particle by the Parkinsonism-associated proteins FBXO7 and PI31

This study reveals how FBXO7 and PI31 regulate the proteasome 20S core particle and how disease variants disrupt this function.

Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1

The paper identifies LRRC58 as a cysteine-responsive E3 ligase that regulates CDO1 stability via ubiquitylation, revealing a mechanism linking cysteine availability to proteasomal turnover.

Charged Molecular Glue Discovery Enabled by Targeted Degron Display

The paper discovers a charged molecular glue, c-Glue, through targeted degron display, enabling the development of Molecular Glue Degraders (MGDs) for protein degradation.

A CK2-FBXW11 kinase-E3 ubiquitin ligase cascade is a metabolic sensor regulating Tryptophan 2,3-dioxygenase stability

The paper identifies a CK2-FBXW11 kinase-E3 ubiquitin ligase cascade that regulates TDO2 stability through Trp-dependent phosphorylation shielding.

Monovalent Pseudo-Natural Product Degraders Supercharge the Native Degradation of IDO1 by KLHDC3

The paper identifies iDegs, which enhance IDO1 degradation via the native CRL2KLHDC3 pathway, reducing kynurenine formation and offering new therapeutic potential for cancer.

The E3 ubiquitin ligase mechanism specifying target-directed microRNA degradation

This paper identifies how a human AGO-miRNA-trigger complex binds ZSWIM8 for CUL3-mediated polyubiquitylation, leading to target-directed miRNA degradation.

Structural basis of an EMC:Spf1 insertase-dislocase complex in the eukaryotic endoplasmic reticulum

The paper presents a cryo-EM structure of the EMC:Spf1 supercomplex, showing how it integrates TMD insertion and dislocation activities for membrane protein quality control.

Employing neutron-encoded monoUbs to study E2/E3 ligase activity and selectivity for assembling Ub chains

The paper develops a mass spectrometry-based assay to screen E2/E3 ligase pairs for their ability to form ubiquitin chains and analyze chain types.

Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST

This study uses nMOST to profile proteo-lipidomics in lysosomal storage disease mutants, revealing autophagy defects and mitochondrial dysfunction.

The structural context of PTMs at a proteome wide scale

The paper determines the structural context of PTMs across the human proteome and identifies regulatory sites by analyzing their distribution in folded and disordered regions.

Data-independent acquisition method for ubiquitinome analysis reveals regulation of circadian biology

The paper develops a DIA-based workflow for ubiquitinome analysis, revealing circadian regulation through novel ubiquitination sites and clusters.

DIA-based systems biology approach unveils novel E3-dependent responses to a metabolic shift

The paper introduces a DIA-based systems biology workflow for high-throughput proteome analysis in yeast, revealing novel E3 ligase responses to metabolic shifts.

Integrative proteomics reveals principles of dynamic phospho-signaling networks in human erythropoiesis

This study uses proteomics to analyze dynamic phospho-signaling networks in human erythropoiesis, identifying key regulatory mechanisms and markers across differentiation stages.

Interconversion between Anticipatory and Active GID E3 Ubiquitin Ligase Conformations via Metabolically Driven Substrate Receptor Assembly

The paper reveals how environmental changes trigger structural transitions in GID E3 ubiquitin ligase complexes, enabling substrate recognition and degradation.