Monika Mehta

The paper develops hypercleavage TOP2 mutants in yeast, human, and mouse to study TOP2-mediated DNA damage and toxicity, showing their utility in genetic screens and in vivo models.

The paper establishes reference samples with high-coverage whole-genome sequencing to create truth sets for somatic mutation and germline variant detection using NGS technologies.

This study benchmarks scRNA-seq reproducibility across platforms and pipelines, finding that batch correction methods significantly affect results.

The paper identifies artefactual fusion DNA sequences produced by blunt-end adapter ligation in PacBio library preparation and recommends an alternative A/T overhang method to reduce them.