Yaojun Tong

The paper develops a method to efficiently clone large GC-rich BGCs from Streptomyces genomes using a CRISPR/Cas12a-based system, demonstrating its effectiveness with a 87-kb BGC.

The paper develops CRISPR-BEST, a DSB-free base editing system for filamentous actinomycetes, achieving single-nucleotide resolution in Streptomyces species.

This paper introduces a yeast platform for producing bacterial aromatic polyketides using a synthetic polyketide synthase system.