Simple cloning of large natural product biosynthetic gene clusters from Streptomyces by an engineered CRISPR/Cas12a system
Mindong Liang, Leshi Liu, Fei Xu, Xiaoqian Zeng, Ruijun Wang, Yang Jinling, Weishan Wang, Loganathan Karthik, Jiakun Liu, Zhiheng Yang, Guoliang Zhu, Shuliu Wang, Linquan Bai, Yaojun Tong, Xueting Liu, , Gao‐Yi Tan
The paper develops a method to efficiently clone large GC-rich BGCs from Streptomyces genomes using a CRISPR/Cas12a-based system, demonstrating its effectiveness with a 87-kb BGC.
Nucleic Acids Research · Mar 10, 2022Peer-reviewedSynthetic Biology