Hana Kiyama

Haloplasma MreB reconstitution in a minimal synthetic bacterium reveals orthogonal force directions underlying distinct Mollicute motility systems

The paper expresses MreB isoforms from Haloplasma in a synthetic bacterium to study motility, finding distinct movement patterns and force directions.

Coordinated Membrane Deformation Driven by a Minimal Set of Spiroplasma MreB Isoforms

The paper investigates how Spiroplasma MreB isoforms deform membranes, showing that SpeMreB5 drives deformation while SpeMreB4 suppresses it, with nucleotide state affecting the process.

Live imaging of bacterial actin MreBs from Spiroplasma causing helicity switching of a minimal synthetic cell

The paper investigates the helicity switching mechanism of Spiroplasma MreB proteins using fluorescent labeling and inhibitors, finding that MreB5 dynamics are static during movement and sensitive to A22.

A highly active bacterial actin actuates the polymerization of another isoform essential for swimming motility of Spiroplasma

This study identifies SpeMreB1 as the most active MreB isoform in Spiroplasma, which manages SpeMreB5 polymerization to drive swimming motility.

Rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through Cre/loxP system

The paper develops a rapid in vitro method to transfer DNA fragments into the JCVI-syn3B genome using the Cre/loxP system, overcoming issues with E. coli-based methods.

Reconstitution of minimal motility system based on Spiroplasma swimming by expressing two bacterial actins in synthetic minimal bacterium

The paper demonstrates that expressing two bacterial actins in a synthetic minimal bacterium enables swimming motility, suggesting that bacterial actins can generate helical cell shapes and movement.