Makoto Miyata

This study isolates two Hodarchaeales strains, closely related to the eukaryotic common ancestor, revealing their syntrophic, anaerobic, and oxygen-tolerant physiology and genomic features.

The paper expresses MreB isoforms from Haloplasma in a synthetic bacterium to study motility, finding distinct movement patterns and force directions.

The paper investigates how Spiroplasma MreB isoforms deform membranes, showing that SpeMreB5 drives deformation while SpeMreB4 suppresses it, with nucleotide state affecting the process.

The paper investigates the helicity switching mechanism of Spiroplasma MreB proteins using fluorescent labeling and inhibitors, finding that MreB5 dynamics are static during movement and sensitive to A22.

This study identifies SpeMreB1 as the most active MreB isoform in Spiroplasma, which manages SpeMreB5 polymerization to drive swimming motility.

This study investigates the function of FtsZ in a cell wall-less bacterium, showing that SeFtsZ forms ring-like structures in cell wall-less cells, suggesting a role in cell division independent of cell wall synthesis.

The paper presents a 3.2 Å resolution cryo-EM structure of a dimeric F1-like ATPase complex in Mycoplasma mobile, revealing its role in gliding motility through conserved rotary mechanisms.

This study visualizes peptidoglycan layers in gliding diderm bacteria using electron microscopy, revealing structural differences that may explain force transmission during locomotion.

This study demonstrates light-controlled actin polymerization in protocells, enabling directed motility and revealing the role of branched and linear actin networks in membrane protrusion.

The paper develops a rapid in vitro method to transfer DNA fragments into the JCVI-syn3B genome using the Cre/loxP system, overcoming issues with E. coli-based methods.

This study uses quick-freeze deep-etch electron microscopy to visualize Bacillus subtilis spore structure and germination, identifying five distinct features during the process.

This study uses electron tomography to analyze the internal structure of Mycoplasma mobile gliding machinery, revealing motor complexes and structural components involved in gliding.

The paper reports the cryo-EM structure of a bacterial adhesin complex and identifies epitopes that affect adhesion and motility in Mycoplasma pneumoniae.

The paper demonstrates that expressing two bacterial actins in a synthetic minimal bacterium enables swimming motility, suggesting that bacterial actins can generate helical cell shapes and movement.

The paper analyzes MreB sequences from Spiroplasma to determine their phylogenetic origin and sequence features, identifying key differences from Bacillus MreBs that may relate to their unique swimming mechanism.