Jesse M. Gray

A prime-editing recorder in one mouse embryo yields a dated, zygote-rooted lineage tree of 1.28M cells at E13.5, enabling clonal dominance, sibling-fate and cell-type coupling analyses.

The paper designs and characterizes diverse genomic parts for precise genome editing and molecular recording in mammalian cells.

The paper shows that firing rate homeostasis in cultured mouse neurons occurs without activity-regulated transcription, challenging the hypothesis that transcription is required for this process.

The paper shows blue light induces neuronal-activity-regulated gene expression in cultured mouse neurons without optogenetic proteins, suggesting blue light is unsuitable for long-term optogenetic experiments measuring transcription.

The paper shows that distinct neuronal activity patterns induce different gene expression programs through specific mechanisms.