Author

Jason T. Fontana

University of WashingtonORCID 0000-0003-4758-6494Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Approach:experimental assay· 2 Research Focus:perturbation response prediction· 2 Organism:bacteria (other)· 1 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1 Approach:statistical model· 1

Frequent Co-Authors

Cholpisit KiattiseweeiD· 2 shared James M. CarothersiD· 2 shared Jesse G. ZalataniD· 2 shared Aria WallsiD· 1 shared Benjamin I. TickmaniD· 1 shared Chen DongiD· 1 shared David Sparkman-Yager· 1 shared Héctor García MartíniD· 1 shared

Activity by Year

2020-2024 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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2 shared papersSynthetic Biology

Cholpisit KiattiseweeORCID

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2 papers

Recent·Most cited

Synthetic BiologyNature CommunicationsPeer-reviewedJul 27, 2024

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling

Jason T. Fontana, David Sparkman-Yager, Ian Faulkner…·James M. Carothers

The paper designs guide RNA structures for CRISPRa to optimize multi-gene expression in bacterial metabolic pathways, achieving higher product titers and identifying pathway bottlenecks.

30 citationstop 4%

E. coliCRISPR screen

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Synthetic BiologyNature CommunicationsPeer-reviewedApr 1, 2020

Effective CRISPRa-Mediated Control of Gene Expression in Bacteria Must Overcome Strict Target Site Requirements

Jason T. Fontana, Chen Dong, Cholpisit Kiattisewee…·Jesse G. Zalatan

This study identifies strict target site requirements for effective CRISPRa in bacteria and demonstrates a dCas9 variant with expanded PAM specificity to overcome these limitations.

105 citationstop 3%

bacteria (other)CRISPR screen

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