Author

Ian Faulkner

University of WashingtonORCID 0009-0009-7715-0908Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:E. coli· 3 Approach:experimental assay· 3 Assay:CRISPR screen· 2 Research Focus:drug response / drug discovery· 2 Research Focus:perturbation response prediction· 2 Research Focus:gene regulatory network inference· 1 Approach:statistical model· 1

Frequent Co-Authors

James M. CarothersiD· 3 shared Jesse G. ZalataniD· 3 shared Cholpisit KiattiseweeiD· 2 shared Ryan A. L. CardiffiD· 2 shared Aria WallsiD· 1 shared Brian H DarstiD· 1 shared David Sparkman-Yager· 1 shared Héctor García MartíniD· 1 shared

Activity by Year

2024-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 34 mutual co-author links

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3 shared papersSynthetic Biology

James M. CarothersORCID

Mutual co-authors in cluster

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3 papers

Recent·Most cited

Synthetic BiologybioRxivPreprintJun 3, 2026

CRISPRi-assisted E. coli strains increase success rate of burdensome construct cloning

Ian Faulkner, Cholpisit Kiattisewee, Brian H Darst…·James M. Carothers

The paper introduces CRISPRi-assisted E. coli strains to improve cloning success for burdensome genetic constructs by reducing metabolic burden during cloning.

DOI 10.64898/2026.06.02.729553

E. coliexperimental assay

𝕏 PostBluesky

Synthetic BiologyNature CommunicationsPeer-reviewedJul 27, 2024

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling

Jason T. Fontana, David Sparkman-Yager, Ian Faulkner…·James M. Carothers

The paper designs guide RNA structures for CRISPRa to optimize multi-gene expression in bacterial metabolic pathways, achieving higher product titers and identifying pathway bottlenecks.

30 citationstop 4%

E. coliCRISPR screen

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Synthetic BiologyNucleic Acids ResearchPeer-reviewedApr 3, 2024

CRISPR-Cas tools for simultaneous transcription & translation control in bacteria

Ryan A. L. Cardiff, Ian Faulkner, Juliana G Beall…·Jesse G. Zalatan

The paper develops CRISPR-Cas tools to independently control transcription and translation in E. coli, showing reduced polar effects with dCas13 and enabling multiplexed gene regulation for biosynthesis.

24 citations

E. coliCRISPR screen

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