Extracellular vesicle cross-linking coupled with solid-phase purification facilitates discovery of ribonucleoprotein complexes assembled on EV RNAs.
Rajat Dhyani, Stephen Clarke, Masashi Kuroda…·Manuel Ascano
This paper introduces two methods, EV-CLASP and EV(r)-CLASP, to study RNP complexes on EV RNAs, revealing post-transfer interactions and intra-vesicular compositions.