Author

Harshad D. Vishwasrao

National Institutes of HealthORCID 0009-0004-3188-1012Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:mouse· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell-cell communication· 1 Approach:experimental assay· 1 Assay:fluorescence / live-cell microscopy· 1 Assay:imaging· 1 Approach:microscopy & imaging analysis· 1

Frequent Co-Authors

Andrius MasedunskasiD· 1 shared Desu CheniD· 1 shared Edna C. HardemaniD· 1 shared Hari ShroffiD· 1 shared Inna A. BelyantsevaiD· 1 shared Jiji CheniD· 1 shared Junko MiyoshiiD· 1 shared Kedar NarayaniD· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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1 shared paperCell Biology

Andrius MasedunskasORCID

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2 papers

Recent·Most cited

Intravital single-molecule microscopy reveals protein kinetics in live mammalian tissues

Marco Heydecker, Desu Chen, Andrius Masedunskas, Melissa R. Mikolaj, Kedar Narayan, Jiji Chen, Harshad D. Vishwasrao, Tobias Meckel, Edna C. Hardeman, Peter William Gunning, Roberto Weigert

This paper introduces iSiMM, a method for tracking single molecules in live tissues, and demonstrates its application to study protein dynamics in mice.

bioRxiv · Oct 31, 2026 · PreprintCell Biology

Live-cell single-molecule fluorescence microscopy for protruding organelles reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of inner ear hair cells

Takushi Miyoshi, Harshad D. Vishwasrao, Inna A. Belyantseva, Junko Miyoshi, Mrudhula Sajeevadathan, Yasuko Ishibashi, Samuel Mawuli Adadey, Narinobu Harada, Hari Shroff, Thomas B. Friedman

This study uses live-cell single-molecule fluorescence microscopy to investigate MYO7A trafficking in stereocilia, revealing its dimeric form and movement restrictions mediated by plasma membrane and F-actin interactions.

Nature Communications · Sep 1, 2025Peer-reviewedCell Biology