Melissa R. Mikolaj

This paper introduces iSiMM, a method for tracking single molecules in live tissues, and demonstrates its application to study protein dynamics in mice.

This study identifies hepatobiliary progenitor-like reprogramming in liver metastases, driven by TGF-β and hypoxia, and shows its association with poor survival.

The paper identifies a temperature- and lipid saturation-dependent transformation of ER membranes into rigid tubes under stress, driven by lipid demixing and protein segregation.

The paper develops NucleoNet and DropNet, deep learning models for segmenting nuclei and lipid droplets in EM images, and applies them to compare 2D/3D cancer models with in vivo tumors.

This paper compares two 3D culture models for breast cancer cells, finding that the EmC model better mimics lung metastasis by altering metabolism and gene expression.

This study identifies and characterizes specialized endothelial cells in zebrafish gills that resemble mammalian lung aerocytes, using single-cell RNA sequencing and advanced imaging techniques.

The paper uses optogenetic perturbation and electron microscopy to show structural diversity in zebrafish left-right organizer cilia and their role in morphogenesis.