Mapping protein-metabolite interactions in E. coli by integrating chromatographic techniques and co-fractionation mass spectrometry.
Mateusz Maciej Wagner, Ji-Eun Kang, Catherine Mercado, Venkatesh P. Thirumalaikumar, Michał Górka, Hanne Zillmer, Jingzhe Guo, Romina I. Minen, Caroline F Plecki, Katayoon Dehesh, Frank C. Schroeder, Dirk Walther, Aleksandra Skirycz
The paper introduces PROMIS, a co-fractionation mass spectrometry technique integrated with chromatographic methods to map protein-metabolite interactions in E. coli, identifying 994 interactions and revealing novel functional insights.
iScience · May 9, 2025Peer-reviewedBiochemistry