Sebastian Palacios

This study investigates how DNA methylation and H3K9me3 maintain transgene silencing and shows that targeted DNA demethylation can reverse it in mammalian cells.

This study reveals analog epigenetic memory through graded DNA methylation, showing its temporal stability and role in gene expression regulation.

The paper identifies OCT4 trajectories critical for reprogramming human fibroblasts to iPSCs and develops a synthetic genetic circuit to enforce these trajectories, improving reprogramming efficiency.

The paper introduces PERSIST, a CRISPR-based RNA regulation platform that enables programmable gene circuit design through modular RNA-level switches, showing resistance to silencing compared to transcriptional regulation.