Nika Shakiba

The paper describes a cost-effective E. coli-based method for producing endotoxin-free cytokines, demonstrating bioactivity comparable to commercial products at 5%-10% of the cost.

The paper develops a framework to predict and control collective fate in multicellular systems by identifying a control regime where a single guide cell can redirect tissue-level organization.

The paper identifies OCT4 trajectories critical for reprogramming human fibroblasts to iPSCs and develops a synthetic genetic circuit to enforce these trajectories, improving reprogramming efficiency.

This paper introduces CloneSelect, a multi-kingdom genetic barcoding system that enables precise isolation of target clones using CRISPR base editing to trigger reporter gene expression.

The paper develops an in vitro platform to study fate patterning in hPSCs, revealing Nodal-dependent switches between gastrulation and neurulation-associated gene profiles.

The paper develops a high-throughput assay to study peri-gastrulation-like fate patterning in human pluripotent stem cells, revealing coordination between Reaction-Diffusion and Positional-Information mechanisms.