Lauren M. Kuo

A prime-editing recorder in one mouse embryo yields a dated, zygote-rooted lineage tree of 1.28M cells at E13.5, enabling clonal dominance, sibling-fate and cell-type coupling analyses.

The paper introduces ArgiNLS, a nuclear localization signal that improves image segmentation of single-cells by restricting fluorescent proteins to the nucleus, enhancing machine learning-based segmentation.

This paper develops a method to efficiently insert fluorescent tags into genes not expressed in hiPSCs using AAV6 delivery, optimized CRISPR conditions, and ddPCR/ICE to minimize INDELs and isolate tagged clones.