Andrew R. M. Michael

The paper identifies signal attrition in XL-MS as the main cause of poor interactome sampling, not low crosslink yield, and highlights issues with signal splitting and database search tools.

The paper introduces a novel crosslinking method using click chemistry to enhance XL-MS sampling depth and detect more protein-protein interactions.

This study introduces a formaldehyde-based fixation method that improves in situ XL-MS performance while preserving cellular ultrastructure in human A549 cells.

The paper introduces an upgraded CRIMP tool for detecting crosslinked peptides in XL-MS data, showing improved sensitivity and resolution of protein interactions.