Arthur Macha

This paper presents a protocol for purifying recombinant proteins from Nicotiana benthamiana for structural studies, demonstrating its effectiveness for complex purification and cryo-EM analysis.

This study identifies the molecular mechanism of gephyrin clustering at GABAergic synapses, showing collybistin and phosphoinositides drive high-molecular weight complex formation, while gephyrin phosphorylation at Ser325 disrupts it.

This paper demonstrates that sodium phosphotungstate and ammonium molybdate can replace uranyl-based stains in negative staining electron microscopy for single particle analysis, offering non-radioactive, cost-effective alternatives.