BiochemistrybioRxivPreprint
In vitro reconstitutions suggest a general model for paradoxical activation of ARAF, BRAF, and CRAF by diverse RAF inhibitor types that does not rely on negative allostery.
Emre Tkacik, Dong Man Jang, Kayla Boxer…·Michael J. Eck
This study shows that RAF inhibitors paradoxically activate ARAF, BRAF, and CRAF through dimerization, not negative allostery, by using biochemical assays and mass photometry.
DOI 10.64898/2025.12.15.694443