Lei Huang

PAMPHLET predicts PAM sequences for CRISPR-Cas systems by expanding spacer data through homology searches, achieving high accuracy validated by experimental methods.

CRISPR-Detector detects, visualizes, and annotates genome-wide mutations from CRISPR/Cas gene editing events with improved speed, scalability, and accuracy.

This study identifies specific distal enhancer-promoter associations that can reprogram MEF cells into iPS-like clones, demonstrating a method to generate pluripotent cells through 3D chromatin conformation.

This paper develops CRISPR systems to target and deplete an oncogenic KRAS mutant allele, leading to tumor regression in vitro and in vivo.

The paper investigates how different enhancer-promoter associations influence Sox2 transcription in mouse embryonic stem cells, revealing distinct biological effects based on interaction type.