Shuo Liu

The paper analyzes off-target effects of PAM-relaxed Cas9 editors in rice using whole-genome sequencing and finds ABE8e causes ~500 A-to-G mutations per plant at TA motifs.

CasLocusAnno annotates Cas proteins and loci, classifies them into (sub)types, and outperforms existing tools in accuracy and speed.

The paper validates the principle of co-adaptation between tRNA gene copy number and amino acid usage across three life domains, showing it improves translation efficiency in both organisms and individual proteins.

The paper develops a computational model using sequence information to predict human gene essentiality, achieving high AUC scores and identifying previously overlooked essential genes.