This study develops and evaluates TadA-8e-derived cytosine and dual base editors in plants, showing high efficiency, purity, and specificity with no off-target effects.

Nature Communications · Jun 14, 2024Peer-reviewedMolecular Biology

Genome-wide investigation of multiplexed CRISPR-Cas12a-mediated editing in rice

Yingxiao Zhang, Yuechao Wu, Gen Li, Aileen Qi, Yong Zhang, Tao Zhang, Yiping Qi

The paper investigates off-target effects of multiplexed CRISPR-Cas12a editing in rice using whole-genome sequencing and finds low-frequency chromosomal deletions and duplications with high DSB numbers.

The Plant Genome · Sep 30, 2022Peer-reviewedGenomics

Genome-wide analyses of PAM-relaxed Cas9 genome editors reveal substantial off-target effects by ABE8e in rice

Yuechao Wu, Qiurong Ren, Zhaohui Zhong, Guanqing Liu, Yangshuo Han, Yu Bao, Li Liu, Shuyue Xiang, Shuo Liu, Xu Dong Tang, Jianping Zhou, Xuelian Zheng, Simon Sretenovic, Tao Zhang, Yiping Qi, Yong Zhang

The paper analyzes off-target effects of PAM-relaxed Cas9 editors in rice using whole-genome sequencing and finds ABE8e causes ~500 A-to-G mutations per plant at TA motifs.

Plant Biotechnology Journal · May 7, 2022Peer-reviewedGenetics