This paper describes a drop on fixed target method for time-resolved serial crystallography, tested with enzymes to improve measurement consistency across facilities.

IUCrJ · May 19, 2026Peer-reviewedBiophysics

Time-resolved ligand dynamics revealed in a β-lactamase using room-temperature serial crystallography

Philip Hinchliffe, Catherine L. Tooke, Michael Beer, Pierre Aller, Jos J. A. G. Kamps, Laura Parkinson, Tiankun Zhou, N. E. Devenish, Do-Heon Gu, Anastasya Shilova, Matthew J. Rodrigues, Abbey J. Telfer, Agata Butryn, Marko Hanževački, Emily I. Freeman, , JAMES M. SPENCER

This study uses room-temperature serial crystallography to observe time-dependent ligand dynamics of the β-lactamase CTX-M-15 with avibactam, revealing conformational changes critical for inhibitor reformation.

bioRxiv · Dec 19, 2025 · PreprintBiochemistry

Droplet on Demand Tape Drive and XES Prototypes for Time-Resolved Serial Crystallography at VMXi, Diamond Light Source

Pierre Aller, Juan Sánchez-Weatherby, Abbey J. Telfer, Robert Bosman, N. E. Devenish, Philip Hinchliffe, Sam Horrell, Jophy Ip, Richard Littlewood, Andrew Male, Eva Giminez Navarro, Urszula Neuman, Jos J. A. G. Kamps, David Omar, Laura Parkinson, , Allen Milster Orville

The paper demonstrates proof of concept for combined droplet on demand serial crystallography and XES experiments at a synchrotron beamline.

bioRxiv · Nov 19, 2025 · PreprintBiophysics