Shailab D. Shrestha

Defining the order of assembly of the Clostridioides difficile divisome complex

This study defines the hierarchical order of divisome assembly in C. difficile using CRISPRi and fluorescent fusions, revealing a unique division mechanism distinct from model bacteria.

Functional redundancy between penicillin-binding proteins during asymmetric cell division in Clostridioides difficile

The paper shows that SpoVD's catalytic activity is dispensable for asymmetric division in C. difficile due to PBP3's compensatory role, suggesting functional redundancy among bPBPs.