Gregory A. Harrison

This study defines the hierarchical order of divisome assembly in C. difficile using CRISPRi and fluorescent fusions, revealing a unique division mechanism distinct from model bacteria.

The paper shows that SpoVD's catalytic activity is dispensable for asymmetric division in C. difficile due to PBP3's compensatory role, suggesting functional redundancy among bPBPs.

This study identifies novel features of the non-canonical divisome complex in C. difficile, revealing an essential regulatory domain in PBP1 and an unexpected role for PBP3 in cell division.